Generator

Part:BBa_K2201332:Design

Designed by: Svenja Vinke   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


NM-domain of Sup35 with an cysteine at position 21 and 121 and histidine tag.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The codons for the cysteines were inserted via site directed mutagenesis and the T7 promoter via bio brick assembly.


Source

BBa_K2201231

References